One of our goals with SalivaDirect is to test a number of alternate reagents, and combinations of alternate reagents, at each step. This will add some redundancy and flexibility in the supply chain (NEB backed up on kit orders? Order from Thermo!) as well as allow labs to substitute whatever reagents they have or have access to.

Tested alternate reagents

Proteinase K

RT-qPCR Kit

RT-qPCR instrument